Last data update: Apr 29, 2024. (Total: 46658 publications since 2009)
Records 1-8 (of 8 Records) |
Query Trace: Rungsrisuriyachai K[original query] |
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Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras.
Quaye O , Roy S , Rungsrisuriyachai K , Esona MD , Xu Z , Tam KI , Banegas DJC , Rey-Benito G , Bowen MD . Mem Inst Oswaldo Cruz 2018 113 (1) 9-16 BACKGROUND: Although first detected in animals, the rare rotavirus strain G10P[14] has been sporadically detected in humans in Slovenia, Thailand, United Kingdom and Australia among other countries. Earlier studies suggest that the strains found in humans resulted from interspecies transmission and reassortment between human and bovine rotavirus strains. OBJECTIVES: In this study, a G10P[14] rotavirus genotype detected in a human stool sample in Honduras during the 2010-2011 rotavirus season, from an unvaccinated 30-month old boy who reported at the hospital with severe diarrhea and vomiting, was characterised to determine the possible evolutionary origin of the rare strain. METHODS: For the sample detected as G10P[14], 10% suspension was prepared and used for RNA extraction and sequence independent amplification. The amplicons were sequenced by next-generation sequencing using the Illumina MiSeq 150 paired end method. The sequence reads were analysed using CLC Genomics Workbench 6.0 and phylogenetic trees were constructed using PhyML version 3.0. FINDINGS: The next generation sequencing and phylogenetic analyses of the 11-segmented genome of the G10P[14] strain allowed classification as G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. Six of the genes (VP1, VP2, VP3, VP6, NSP2 and NSP4) were DS-1-like. NSP1 and NSP5 were AU-1-like and NSP3 was T6, which suggests that multiple reassortment events occurred in the evolution of the strain. The phylogenetic analyses and genetic distance calculations showed that the VP7, VP4, VP6, VP1, VP3, NSP1, NSP3 and NSP4 genes clustered predominantly with bovine strains. NSP2 and VP2 genes were most closely related to simian and human strains, respectively, and NSP5 was most closely related to a rhesus strain. MAIN CONCLUSIONS: The genetic characterisation of the G10P[14] strain from Honduras suggests that its genome resulted from multiple reassortment events which were possibly mediated through interspecies transmissions. |
Molecular characterization of a human G20P[28] rotavirus a strain with multiple genes related to bat rotaviruses.
Esona MD , Roy S , Rungsrisuriyachai K , Gautam R , Hermelijn S , Rey-Benito G , Bowen MD . Infect Genet Evol 2017 57 166-170 Group A rotaviruses are the major cause of severe gastroenteritis in the young of mammals and birds. This report describes characterization of an unusual G20P[28] rotavirus strain detected in a 24month old child from Suriname. Genomic sequence analyses revealed that the genotype constellation of the Suriname strain RVA/Human-wt/SUR/2014735512/2013/G20P[28] was G20-P[28]-I13-R13-C13-M12-A23-N13-T15-E20-H15. Genes VP1, VP2, VP3, NSP1, NSP2, NSP3, NSP4 and NSP5 were recently assigned novel genotypes by the Rotavirus Classification Working Group (RCWG). Three of the 11 gene segments (VP7, VP4, VP6) were similar to cognate gene sequences of bat-like human rotavirus strain Ecu534 from Ecuador and the VP7, NSP3 and NSP5 gene segments of strain RVA/Human-wt/SUR/2014735512/2013/G20P[28] were found to be closely related to gene sequences of bat rotavirus strain 3081/BRA detected in Brazil. Although distantly related, the VP1 gene of the study strain and bat strain BatLi09 detected in Cameroon in 2014 are monophyletic. The NSP1 gene was found to be most closely related to human strain QUI-35-F5 from Brazil. These findings suggest that strain RVA/Human-wt/SUR/2014735512/2013/G20P[28] represents a zoonotic infection from a bat host. |
Characterization of a triple-recombinant, reassortant rotavirus strain from the Dominican Republic.
Esona MD , Roy S , Rungsrisuriyachai K , Sanchez J , Vasquez L , Gomez V , Rios LA , Bowen MD , Vazquez M . J Gen Virol 2017 98 (2) 134-142 We report the genome of a novel human triple-recombinant G4P[6-8_R] mono-reassortant strain identified in a stool sample from the Dominican Republic during routine facility-based rotavirus strain surveillance. The strain was designated as RVA/Human-wt/DOM/2013840364/2013/G4P[6-8_R], with a genomic constellation of G4-P[6-8_R]-I1-R1-C1-M1-(A1-A8_R)-N1-(T1-T7_R)-E1-H1. Recombinant gene segments NSP1 and NSP3 were generated as a result of recombination between genogroup 1 rotavirus A1 human strain and a genotype A8 porcine strain and between genogroup 1 rotavirus T1 human strain and a genotype T7 bovine strain, respectively. Analyses of the RNA secondary structures of gene segment VP4, NSP1 and NSP3 showed that all the recombinant regions appear to start in a loop (single-stranded) region and terminate in a stem (double-stranded) structure. Also, the VP7 gene occupied lineage VII within the G4 genotypes consisting of mostly porcine or porcine-like G4 strains, suggesting the occurrence of reassortment. The remaining gene segments clustered phylogenetically with genogroup 1 strains. This exchange of whole or partial genetic materials between rotaviruses by recombination and reassortment contributes directly to their diversification, adaptation and evolution. |
Molecular characterization of the first G24P[14] rotavirus strain detected in humans.
Ward ML , Mijatovic-Rustempasic S , Roy S , Rungsrisuriyachai K , Boom JA , Sahni LC , Baker CJ , Rench MA , Wikswo ME , Payne DC , Parashar UD , Bowen MD . Infect Genet Evol 2016 43 338-42 Here we report the genome of a novel rotavirus A (RVA) strain detected in a stool sample collected during routine surveillance by the Centers for Disease Control and Prevention's New Vaccine Surveillance Network. The strain, RVA/human-wt/USA/2012741499/2012/G24P[14], has a genomic constellation of G24-P[14]-I2-R2-C2-M2-A3-N2-T9-E2-H3. The VP2, VP3, VP7 and NSP3 genes cluster phylogenetically with bovine strains. The other genes occupy mixed clades containing animal and human strains. Strain RVA/human-wt/USA/2012741499/2012/G24P[14] most likely is the product of interspecies transmission and reassortment events. This is the second report of the G24 genotype and the first report of the G24P[14] genotype combination in humans. |
G2P[4]-RotaTeq Reassortant Rotavirus in Vaccinated Child, United States.
Roy S , Rungsrisuriyachai K , Esona MD , Boom JA , Sahni LC , Rench MA , Baker CJ , Wikswo ME , Payne DC , Parashar UD , Bowen MD . Emerg Infect Dis 2015 21 (11) 2103-4 Group A rotaviruses (RVAs) are a leading cause of acute gastroenteritis-associated deaths among children <5 years of age in developing countries (1). The genome of RVA consists of 11 double-stranded RNA segments that code for 11 or 12 viral proteins (VP1–VP4, VP6, VP7, nonstructural protein 1 [NSP1]–NSP5/6) (2). In 2008, the Rotavirus Classification Working Group established a system of extended classification that was based on the sequences of all 11 gene segments and used the notations Gx-P[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx for the genes VP7, VP4, VP6, VP1–VP3, NSP1–NSP5, respectively (3). Similar to other RNA viruses, RVAs show high genomic diversity, which is generated primarily through point mutations, reassortment, rearrangement, and recombination events. | In 2006 and 2008, two live-attenuated vaccines, RotaTeq (Merck, Whitehouse Station, NJ, USA) and Rotarix (GlaxoSmithKline, Rixensart, Belgium), respectively, were introduced in the United States (4). RotaTeq is a pentavalent human bovine reassortant vaccine that contains 4 G types (G1, G2, G3, and G4; VP7 gene) plus the P[8] VP4 type on a bovine WC3 (G6P[5]) backbone (5). In 2012, Bucardo et al. reported finding a vaccine-derived nonstructural protein 2 (NSP2) gene in 2 wild-type RVA strains with a G1P[8] genogroup 1 backbone (6). Each of these strains had been found during routine surveillance in Nicaragua, where RotaTeq was introduced in 2006, suggesting reassortment of the vaccine strain with circulating wild-type strains. The authors also examined alignments of the NSP2 gene and found no differences at functional domains between the vaccine-derived NSP2 and the circulating wild-type NSP2 (6). This finding could explain why a vaccine-derived NSP2 reassortant was viable. |
Full-Genome Sequence of the First G8P[14] Rotavirus Strain Detected in the United States.
Mijatovic-Rustempasic S , Roy S , Sturgeon M , Rungsrisuriyachai K , Reisdorf E , Cortese MM , Bowen MD . Genome Announc 2015 3 (3) This is a report of the complete genomic sequence of a rare rotavirus group A G8-P[14]-I2-R3-C2-M2-A3-N2-T6-E2-H3 strain detected in a stool sample from a 57-year-old subject. |
Outbreak of Gastroenteritis in Adults Due to Rotavirus Genotype G12P[8].
Pacilli M , Cortese MM , Smith S , Siston A , Samala U , Bowen MD , Parada JP , Tam KI , Rungsrisuriyachai K , Roy S , Esona MD , Black SR . Clin Infect Dis 2015 61 (4) e20-5 BACKGROUND: Rotavirus infection in adults is poorly understood and few rotavirus outbreaks among U.S. adults have been reported in the literature. We describe an outbreak due to genotype G12P[8] rotavirus among medical students, faculty and guests who attended a formal dinner event in April 2013. METHODS: A web-based questionnaire was distributed to event attendees. to collect symptom and exposure data. A clinical case was defined as a person who developed diarrhea after attending the formal event. A laboratory-confirmed case was defined as a clinical case who attended the formal event with rotavirus detected in stool by enzyme immunoassay or reverse transcription-PCR assay. RESULTS: Among 334 formal dinner attendees, 136 (41%) completed a web-based questionnaire; 58 (43%) respondents reported illness. Symptom onset ranged from 1 to 8 days, with peak onset 3 days after the event. In addition to diarrhea, predominant symptoms included fever (91%), abdominal pain (84%) and vomiting (49%). The median duration of illness was 2.5 days. Thirteen (22%) of 58 cases sought medical attention; none were hospitalized. Analysis of food exposures among questionnaire respondents did not identify significant associations between any specific food or drink item and illness. Stool specimens were negative for bacterial pathogens by culture, negative for norovirus by RT-PCR; four specimens were positive for rotavirus by EIA or PCR. G12P[8]-R1-C1-M1-A1-N1-T1-E1-H1 was identified as the causative full-genome genotype. CONCLUSION: Rotavirus outbreaks can occur among adults, including young adults. Health professionals should consider rotavirus as a cause of acute gastroenteritis in adults. |
Full-Genome Sequence of a Rare Human G3P[9] Rotavirus Strain.
Mijatovic-Rustempasic S , Roy S , Sturgeon M , Rungsrisuriyachai K , Esona MD , Degroat D , Qin X , Cortese MM , Bowen MD . Genome Announc 2014 2 (2) This is a report of the complete genomic sequence of a rare rotavirus group A G3-P[9]-I2-R2-C2-M2-A3-N2-T1-E2-H3 strain designated RVA/Human-wt/USA/12US1134/2012/G3P[9]. |
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